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Now you can take 1 mL of that solution, add it to a new container, top off with 99mLs of water, and you ll have only 1/100th of the original bacteria in the.
But the exact same thing would happen if our sample started with 183,001 cells.
I need a hint : 11 of the little bags would go into one big bag I think I have the answer: about 35 11 about 400.
He was giddy with emotion, but realized that convincing his mother would take some work.Let s look at some other ways we could count the same 183,000 cell sample: Dilution Factor plate count resolution ease of counting 00 1830 /-.027 nope, can t do it 183 /- 500.27 boring but do-able 18 /- 5000.7 easy.Serial Dilution Introduction: The Purpose of serial dilution is to determine the number of bacteria per unit volume in the origi-nal culture, determination of the.If your original solution contained 50,000,000 bacteria: the,000 sample will contain about 5,000 CFUs.Basically, it s awfully hard to divide something into teeny-tiny pieces unless you first blow tom clancy's ghost recon future soldier skidrow 1.2 that something up and make it huge.That would be pretty difficult with a pipette.The prince passed calculus, graduated with slight honors, and lived out his life in bitter isolation.But really, a single 1/10th dilution would suffice.First of all, we re going to use the Viable Plate Count method - the only method that can tell a live cell from a dead one for a rundown on counting methods, look here.But on the other hand, how can we avoid.So the real trick here is picking the correct dilution.
So you can see that the 30 to 300 guideline is really a compromise between countability and error.


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